diff --git a/freqtrade/optimize/hyperopt.py b/freqtrade/optimize/hyperopt.py
index fcf50af6a..f9dddd573 100644
--- a/freqtrade/optimize/hyperopt.py
+++ b/freqtrade/optimize/hyperopt.py
@@ -624,6 +624,7 @@ class Hyperopt:
 
         # We don't need exchange instance anymore while running hyperopt
         self.backtesting.exchange = None  # type: ignore
+        self.backtesting.pairlists = None  # type: ignore
 
         self.trials = self.load_previous_results(self.trials_file)