diff --git a/freqtrade/configuration/config_validation.py b/freqtrade/configuration/config_validation.py
index 4bfd24677..068364884 100644
--- a/freqtrade/configuration/config_validation.py
+++ b/freqtrade/configuration/config_validation.py
@@ -131,3 +131,15 @@ def _validate_whitelist(conf: Dict[str, Any]) -> None:
         if (pl.get('method') == 'StaticPairList'
                 and not conf.get('exchange', {}).get('pair_whitelist')):
             raise OperationalException("StaticPairList requires pair_whitelist to be set.")
+
+        if pl.get('method') == 'StaticPairList':
+            stake = conf['stake_currency']
+            invalid_pairs = []
+            for pair in conf['exchange'].get('pair_whitelist'):
+                if not pair.endswith(f'/{stake}'):
+                    invalid_pairs.append(pair)
+
+            if invalid_pairs:
+                raise OperationalException(
+                    f"Stake-currency '{stake}' not compatible with pair-whitelist. "
+                    f"Please remove the following pairs: {invalid_pairs}")
diff --git a/tests/test_configuration.py b/tests/test_configuration.py
index ae85c7493..89ca74afa 100644
--- a/tests/test_configuration.py
+++ b/tests/test_configuration.py
@@ -798,6 +798,12 @@ def test_validate_whitelist(default_conf):
 
     validate_config_consistency(conf)
 
+    conf = deepcopy(default_conf)
+    conf['stake_currency'] = 'USDT'
+    with pytest.raises(OperationalException,
+                       match=r"Stake-currency 'USDT' not compatible with pair-whitelist.*"):
+        validate_config_consistency(conf)
+
 
 def test_load_config_test_comments() -> None:
     """